Fig. 1.

Generation of soybean PLDα1 knockdown lines. Soybean PLDα1KD transgenic plants were generated by stable transformation of soybean cultivar Jack with a soybean PLDα1 RNA interference (RNAi) construct. Transgenic soybean plants were used for analysis. a Construction of soybean PLDα1KD. 1151bp one in forward and the other in reverse and separated by a DNA fragment of Pdk-I gene, is driven by a seed specific promoter for soybean β-conglycinin gene encoding a major seed storage protein in seed, and terminated by a Phaseo gene terminator. b Immunoblotting screening PLDα1 in seeds of transgenic soybean plants 1020 (PLDα1KD1). 1–6 indicate individual plants from transgenic line or wild-type Jack (control). c Immunoblotting detection PLDα1 in seeds of another transgenic soybean line 1048 (PLDα1KD2). 1–3 indicate the individual plants from each transgenic line or wild-type Jack (control). d Immunoblotting of PLDα1 using proteins extracted from leaves and seeds from two PLDα1KD lines1. e PLDα1 activity in two PLDα1KD lines 1 and wild-type Jack line1 (control) developing seeds