Fig. 5.

Expression patterns of FAD genes in PLDα1KD and wild-type developing seeds. a FAD enzymes function in phospholipid desaturation with various PC molecules as preferred substrates. b Expression of FAD genes in soybean developing seeds of different genetic backgrounds under various growth conditions. FAD2, Microsomal Δ12 desaturase; FAD3, Microsomal ω3 desaturase; FAD6, Plastidial Δ12 desaturase. The X-axis numbers indicated each development stage. KD1-h, KD2-h and WT-h: PLDα1 knockdown line 1 and 2 and wild-type jack, respectively, under high temperature and humidity; KD1-n and KD2-n and WT-n: PLDα1 knockdown line 1 and 2 and wild-type Jack, respectively, under normal temperature and humidity. 1–5 indicate different developing stages of seeds corresponding to fresh weights as described in “Materials and methods”. The values are the mean + SD (n = 3). * and **Denote significance at P < 0.05 and P < 0.01, respectively, compared with wild-type Jack (WT) based on Student’s t test