Figure 3.

Comparison of Anti-tumor Activity of A3B1- and FMC63-based CAR T Cells

(A) Detection of chimeric antigen receptor (CAR) expression by western blot. (B) Cytotoxicity assay of CART19 cells versus NALM6 cells after 4 hr of co-culture. Percent target surviving cells, relative to untreated, is shown (mean of 3 experiments ± SEM). (C) Upper panel shows a timeline of experimental design (*Im, bioluminescent image; *Bl, blood sample). Lower panels show bioluminescence images showing disease progression at different days. (D) Detection of tumor (GFP+) cells and CD3+ cells in blood by flow cytometry. Control, control mice (no tumor or T cells were injected).