Figure 1.

Effect of hesperidin on rat chondrocytes viability and H₂O₂-induced reduction. The viability of chondrocytes in different groups was assessed by CCK-8 assay. (A) After adherence, cells were treated with 0, 0.001, 0.01, 0.1, 1, or 10 μg/mL of hesperidin for 2 h. (B) The dose-dependent effects of H₂O₂ on cell viability after 24 h treatment. The 50% inhibition concentration value (IC50) was found to be 230 μM. *p<0.05, compared with the 0 μM H₂O₂ group. (C) Hesperidin (0, 0.001, 0.01, 0.1, 1, or 10 μg/mL) was added for 2 h prior to 24 h treatment with 230 μM H₂O₂. * p<0.05, compared with the H₂O₂ group. All data are expressed as the mean ± standard deviation.