Table 1.

Primers sequence and cycling conditions.

Virus	Gene	Primer sequence	Cycling conditions	Product size
Influenza A(H3N2)	M	F:GACAAGACCAATCCTGTCACYTCTG R:AAGCGTCTACGCTGCAGTCC	950C-2 mins, 950C-15 secs, 600C-1 min, 45 cycles	97 bp
	PB1	F:CCCCTGAATCCATTTGTCAGCCATA R:TAGAGCGGTTCCTCTTGGGA	950C-30 secs, 550C-30 secs, 720C-30 secs, 40 cycles	142 bp
	PB2	F:ATTGCGGCCAGGAACATAGT R:CCAATTTGTGTGCTGTGGCA	950C-30 secs, 550C-30 secs, 720C-30 secs, 40 cycles	89 bp
RSV A2	M	F: GGCAAATATGGAAACATACGTGAA R:TCTTTTTCTAGGACATTGTAYTGAACAG	940C-2 mins, 940C-30 secs, 600C-1 min, 45 cycles	134 bp
	F	F:ATGAACAGTTTAACATTACCAAGT R:GTTTTGCCATAGCATGACAC	950C-2 mins, 950C-15 secs, 600C-1 min, 45 cycles	132 bp
	G	F:CGGCAAACCACAAAGTCACA R:TTCTTGATCTGGCTTGTTGCA	950C-2 mins, 940C-30 secs, 540C-30 secs, 720C-1 min, 720C-10 mins, 40 cycles	43 bp
	NS1	F:ATTTAACTCCCTTGGTTAGAG R:GTGTTAGTGACATTGATTTGC	950C-2 mins, 950C-30 secs, 530C-30 secs, 720C-30 secs, 40 cycles	482 bp
	NS2	F:TATGGCACTTTCCCTATGCCAA R:GGGTGTGTGCTTGGTAGGCT	95 °C-2 mins, 95 °C 30 secs, 58 °C-30 secs, 72 °C-30 secs, 40 cycles