Fitm2 Inhibits the Anti-apoptotic and Anti-atrophic Function of miR-212/132
(A) Expression level of Fitm2 in primary neonatal rat cardiomyocytes transduced with AAV6-control and AAV6-Fitm2 (n = 3 technical replicates). (B and C) Cell size of neonatal rat cardiomyocytes transduced with either AAV6-Empty or AAV6-Fitm2 and transfected with pre-miR-Neg, pre-miR-132, and pre-miR-212 in the presence or absence of doxorubicin. (D) Caspase 3/7 activity in neonatal cardiomyocytes after overexpression of miR-132/212 together with AAV6-control or AAV6-Fitm2 transduction in the presence or absence of doxorubicin. *p < 0.05; **p < 0.01. FC, fold change.