Figure 5.

In Vivo Lipid Panel Analysis of MdaPCSK9

(A–F) B6.Cg-foxn1nu/J nude mice (A and B) and C57B/6J wild-type mice (C and D) were bled at the indicated time points following a single intramuscular administration of 300 μg MdaPCSK9 plasmid. (E and F) C57B/6J wild-type mice were sequentially administered 100 μg MdaPCSK9 plasmid by intramuscular injection. Lipid panel (A, C, and E, non-HDL-C; and B, D, F, total cholesterol) analyses were carried out on mice sera using the VITROS 350 Clinical Chemistry Analyzer. Percent changes were calculated for each day relative to control pVax-1-treated mice. Statistical differences are indicated relative to day 0 of treatment. Data are expressed as ± SEM (n = 5). Statistical differences were measured using two-way ANOVA tests (*p < 0.05, **p < 0.01, and ***p < 0.001; n.s., not significant).