. 2018 Feb 1;1(1):65–74. doi: 10.1089/crispr.2017.0010

Table 1.

Efficiency of multiplex gene editing in the protoplasts of hexaploid wheat

Constructs	Spacer	Total reads analyzed	Mutated reads^a	Proportion of mutated reads^b
pBUN421-GLM	GW2T2	103,076	3,545	0.17
	LPX1T2	63,133	377	0.03
	MLOT1	110,290	7,047	0.32
pBUN421-GW2T2	GW2T2	77,731	6,210	0.40
pBUN421-LPX1T2	LPX1T2	49,108	888	0.09
pBUN421-MLOT1	MLOT1	111,379	5,965	0.27

^{^a}

The examples of mutated reads are shown in Supplementary Figures S5 and S6.

^{^b}

Proportion of mutated reads was normalized using the 20% protoplast transformation efficiency estimated for this experiment.