. 2018 Feb 1;1(1):65–74. doi: 10.1089/crispr.2017.0010

Table 2.

Transmission of CRISPR-Cas9-induced mutations in the progenies of MGE construct-expressing wheat lines

T₀ plants	Genome	TaGW2 genotype^a	TaMLO genotype^a	N.O. of T₁ progenies	N.O. of TaGW2 genotypes^a			N.O. of TaMLO genotypes^a
GLM-1	A	aa	aa	28	0 AA	0 Aa	28 aa	0 AA	0 Aa	28 aa^c
	B	bb	BB		0 BB	0 Bb	28 bb^b	—	—	—
	D	dd	DD		0 DD	0 Dd	28 dd	—	—	—
GLM-2	A	Aa	Aa^d	31	7 AA	17 Aa	7 aa	0 AA^d	10 Aa	21 aa
	B	BB	BB		30 BB	1 Bb^e	0 bb	—	—	—
	D	Dd	DD		10 DD	15 Dd	6 dd	—	—	—

^{^a}

A/a, B/b, and D/d represent loci in different genomes; the uppercase and lowercase stand for wild-type and mutated alleles, respectively.

^{^b}

In GLM-1, the B genome copy of the TaGW2 gene has two different mutation types: 1 bp deletion (b1) and 3 bp deletion (b2; Fig. 2A). The segregation ratio of these two mutations is 10:14:4 (b1b1:b1b2:b2b2).

^{^c}

In GLM-1, the A genome copy of the TaMLO gene has two different mutation types: 1 bp deletion (a1) and 47 bp deletion (a2; Fig. 2A). The segregation ratio of these two mutations is 4:14:10 (a1a1:a1a2:a2a2).

^{^d}

Plant GLM-2 has wild-type reads and two different types of mutated reads (Fig. 2A) in the A genome copy of the TaMLO gene. However, its T₁ progenies do not have homozygous wild-type plants.

^{^e}

New TaGW2 gene alleles in the B genome were induced by the transgenerationally active CRISPR-Cas9.