Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 1;1(1):88–98. doi: 10.1089/crispr.2017.0012

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Philippos Aris Papathanos and Nikolai Windbichler 2018; Published by Mary Ann Liebert, Inc.

This article is available under the Creative Commons License CC-BY-NC (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. Permission only needs to be obtained for commercial use and can be done via RightsLink.

PMC Copyright notice

FIG. 4. — kmer analysis and selection in the Pimperena strain. (A) kmer-CQ versus abundance (in log10 of sum) in both male and female Illumina libraries for all 270 million redkmer-generated kmers colored by chromosomal bins. (B) Redkmer selection plot showing kmer-CQ versus abundance for predicted X-chromosome-specific and abundant kmers (red dots) versus the unsuitable kmers (gray dots). (C) Identification of kmers containing sequences predicted to be suitable for targeting by CRISPR endonucleases showing kmer abundance in the Illumina data (x-axis) versus their occurrence within PacBio data (y-axis). Kmers lacking suitable characteristics for CRISPR (e.g., the absence of a PAM motif) are shown as gray dots, kmers suitable for Cas9 in red, kmers suitable for Cpf1 in blue, and those harboring sequences suitable for both Cas9 and Cpf1 are shown in yellow. (D) Number of kmers containing sequences suitable for CRISPR nuclease platforms.