Figure 6. Effect of CD39⁺ and CD39⁻ Treg on cytokine production by conventional CD4⁺ T cells.

PBMC isolated from buffy coats were enriched for monocytes by immunomagnetic sorting, and for conventional T cells (CD4⁺CD127⁺CD25⁻) and CD39⁺ and CD39- Treg (CD4⁺CD127^loCD25^hi) by flow cytometric cell sorting, and stimulated with anti-CD3 mAb. IFN-β, IL-17A and IL-10 concentration was analyzed by MAGPIX array after two days of culture, with responder cells and sorted CD39⁺ or originally CD39⁻ Treg at a 1:1 ratio (n = 5). Data are expressed as % of the cytokine levels compared to cultures without Treg (A). Individuals with no detectable cytokine levels in the cultures without Treg were excluded. Cytokine levels in cultures with CD39⁺ and originally CD39- Treg are displayed as absolute values for all individuals (B), dotted line indicates lowest detectable concentration. Individuals are consistently color-tagged between graphs.