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. Author manuscript; available in PMC: 2019 Apr 13.
Published in final edited form as: Science. 2018 Mar 8;360(6385):215–219. doi: 10.1126/science.aar7899

Fig. 2. Localization of STT3A, RPN1 and DC2 in ribosome-bound OST.

Fig. 2.

(A) Cryo-EM structure of the active solubilized RTC. Ribosome and P-Site tRNA are shown before, the membrane region including Sec61, TRAP and OST after focused refinement (Fig. S2) low-pass filtered to 4 Å. (B) Zoom on the translocon region omitting TRAP as depicted in (A) (upper panel) or rotated by 90° (lower panel). (C, D) Fitted homology model for mammalian STT3A. Density for phosphate groups in the catalytic center is green. (E) Close up view of the cytosolic RPN1 four-helix bundle binding to the ribosome. (F) Zoom on the Sec61-OST interface with a fitted model for the DC2 TMs.