Figure 2. Standard Quaking Induce Conversion Reaction (SQ).

The components of the SQ (rPrP^C substrate, brain homogenate seed and conversion buffer) are represented. The final conversion products from reactions seeded with vCJD brain homogenate dilutions containing the indicated amounts of PrP^res or Alzheimer’s disease (AD) negative controls are shown. Hamster rPrP^C (residues 23–231) was used as substrate and single amplification round reaction proteinase K (PK)-digested products were analyzed using antiserum R20 (C-terminal epitope). Open circles mark 17-kDa fragments and brackets indicate the lower molecular weight bands (10–13 kDa). Immunoblot adapted from Ref. [44].