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. Author manuscript; available in PMC: 2019 Jan 4.
Published in final edited form as: Top Curr Chem. 2011;305:121–133. doi: 10.1007/128_2011_184

Figure 3. Diagram of Real Time QuIC (RTQ) and comparison of end-point dilution titrations of scrapie brain homogenate by RTQ compared to animal bioassay.

Figure 3.

(Top panel) RTQ analysis of normal and scrapie brain homogenate (BH) dilutions using hamster (90–231) rPrPC as a substrate. (Bottom panel) Comparison of hamster brain homogenate end point dilution titrations by RTQ and animal bioassay. The Spearman-Kärber estimate of the SD50 (i.e. seeding dose giving sufficient Thioflavin T fluorescence in half of the replicate wells) per 2 µl of neat brain tissue is indicated. Graphs adapted from Ref. [62].