Fig. 2.

Mebendazole has no inhibitory effect on FAK and ERK1/2 pathways. (A–C) HUVECs were incubated with or without MBZ (10 μM) for 1 h and then stimulated by VEGF (10 ng/ml) (A) or bFGF (10 ng/ml) (B) for indicated time. HUVECs were cultured with or without MBZ (10 μM) for 24 h in HUVEC medium (C). Cells were harvested and analyzed by Western blotting to determine the phosphorylation of FAK (pTyr³⁹⁷) and ERK1/2 (Thr²⁰²/Tyr²⁰⁴). FAK, ERK1/2 and β-actin was examined as a loading control. The numbers indicate the ratio of p-ERK1/2 (Thr²⁰²/Tyr²⁰⁴)/ERK1/2 or p-FAK (pTyr³⁹⁷)/FAK.