Fig. 1.

Sulfuretin inhibits lipid accumulation and adipocyte differentiation. (A–C) Sulfuretin suppressed adipocyte differentiation in 3T3-L1 preadipocytes. (A) Sulfuretin suppressed lipid accumulation as assessed by Oil red O staining. (B) 3T3-L1 preadipocytes were treated with sulfuretin for seven days during adipocyte differentiation and Pparγ protein levels were measured by Western blotting and (C) mRNA expression levels of Pparγ, Fabp4, C/ebpα, and LPL were measured by real time PCR. Resveratrol (RSV) treated at 20 µM for 7 days was used as a control. (D) Primary bone marrow cells isolated from rat tibiae were treated with sulfuretin (10 µM) for seven days and the expression of Pparγ and C/ebpα mRNA was quantified. Data shown represent means ± SEM. Statistical significance was determined relative to a control by a Student’s t-test (^*p<0.05, ^**p<0.005, ^***p<0.0005).