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. 2018 Aug 22;27(1):107–116. doi: 10.4062/biomolther.2018.090

Fig. 5.

Fig. 5.

Identification of Atf3 as a sulfuretin-induced gene. (A) The temporal induction of Atf3 expression by sufuretin was measured by real-time PCR. Data shown represent the mean ± SD of representative data from three independent experiments. (B) The temporal expression of genes induced by sulfuretin. 3T3-L1 preadipocytes were treated with sulfuretin for the indicated time points and the expression of NRF2 target genes (Ho-1, GCLC, P62, GCLM, Nqo-1, and Osgin) was measured. (C) Induction of Atf3 by sulfuretin in the epididymal white adipose tissue from sulfuretin treated mice. Sulfuretin was treated at a daily dose of 10 mg/kg for eight weeks. Data shown represent the mean ± SEM. Statistically significant differences in gene expressions between the control and sulfuretin-treated HFD-fed obese mice (n=7 per group) were determined by a Student’s t-test (*p<0.05). (D) Induction of Atf3 protein by sulfuretin in the epididymal (eWAT) and inguinal (iWAT) white adipose tissues from sulfuretin-treated HFD-fed obese mice. Sulfuretin was administrated at a daily dose of 10 mg/kg for eight weeks.