Fig. 5.

In vivo blockade of the IL-7 receptor CD127 improves some aspects of experimental fungal asthma severity. a C57BL/6 mice were subjected to experimental fungal asthma and on days 7, 9, 11 and 14, mice received rat anti-mouse IL-7Rα/CD127 or rat IgG2a isotype control intraperitoneally. b, c At 24 h after the last organism challenge (b) CCL17 and (c) CCL22 levels were quantified in clarified lung homogenates by ELISA. The Figures illustrates cumulative data from three independent studies. d At 24 h after the last organism challenge, the lungs were lavaged and unfractionated lung lavage cells cultured for 24 h in the presence of A. fumigatus conidia. IL-22 levels were quantified in clarified co-culture supernatants by ELISA. The Figure illustrates cumulative data from two independent studies. e, f At 24 h after the last organism challenge, lung cells were isolated by bronchoalveolar lavage, enumerated, Fc-blocked and stained with a live/dead staining kit followed by staining with fluorochrome-conjugated antibodies corresponding to (e) total BAL cells and (f) CD4 T cells, γδ T cells, iNKT cells and eosinophils. (g) At 24 h after the last organism challenge, dynamic lung resistance was analyzed via mechanical ventilation using the flexiVent system. The Figure illustrates cumulative data from two independent studies. Data are expressed as mean ± SEM. For all graphs, *, ** and *** represent P values of <0.05, <0.01 and <0.001, respectively; n = 4–6 mice/group for each study; each data point/dot represents a single mouse and the line in each group corresponds to the mean