Table 1. Brains of mice receiving IVT topotecan show inflammation and brain necrosis.

	# of mice/ group	Necrosis	Inflammation
Saline IVT pump	4	0 / 4	0 / 4
Saline IP bolus	8	0 / 8	0 / 8
TPT IP bolus	9	0 / 9	0 / 9
TPT IVT bolus	7	4 / 4 a	5 / 7
TPT IVT pump	6	6 / 6	6 / 6

Summary of findings in the harvested mouse brains, as evaluated by H&E staining of 2–4 FFPE sections from each brain: Numbers in the denominator reflect the number of brains from the group that were assessible for necrosis or inflammation. The numerator reflects the number of brains in which necrosis or inflammation was found. Evaluation was by two independent blinded observers.

^a Brain necrosis and inflammation were only seen in brains with IVT topotecan (bold font). Where necrosis was present, it was in the general region shown in Fig 6. Inflammation and tumor were not specific to this region. In three of the brains of the IVT bolus TPT group, the brains were torn during harvesting such that they were missing the region where necrosis was seen in other IVT TPT mice. It is possible that this tissue loss occurred during removal of the cannulas when preparing the brains for fixation. Therefore, the denominator for evaluation of necrosis in the group of mice receiving IVT topotecan bolus was only 4 of the 7 brains.