Table 2. Inhibitory effects of the alkaloids from Matis Dart Poison and d-TC at different concentrations (IC₅₀ and 3x IC₅₀) on the acetylcholine dose–response curve at mouse adult α1β1εδ nAChR expressed in Neuro2a cells.

Compound	[Ca2+]i rise
	α1β1εδ nAChR
ACh	EC50, μM (95% CI)		nH (mean ± SEM)
	8.04 (5.04–11.04)		2.19 ± 0.41
Compound	IC50 concentration		3x IC50 concentration
	EC50, μM	nH	EC50, μM	nH
	(95% CI)	(mean ± SE)	(95% CI)	(mean ± SE)
ACh + d-TC	33.05	3.90 ± 1.28	65.32	3.11 ± 0.78
	(17.27–48.82)		(41.28–89.36)
ACh + BBIQA1	26.53	0.90 ± 0.05	50.44	1.55 ± 0.34
	(19.83–33.23)		(23.90–76.98)
ACh + BBIQA2	17.63	1.60 ± 0.34	25.22	1.74 ± 0.14
	(8.68–26.58)		(21.15–29.29)