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. 2019 Jan 4;14(1):e0210013. doi: 10.1371/journal.pone.0210013

Fig 4. The mRNA expression of enzymes involved in CLE formation upon IL-4 stimulation.

Fig 4

ALOX12B, ALOXE3 and ABHD9 mRNA levels of primary normal human epidermal keratinocytes were measured by real-time RT-PCR. The mean values of threshold cycle were 27.5 ± 0.5 (ALOX12B), 22.4 ± 0.1 (ALOXE3), 23.1 ± 0.4 (ABHD9), 15.4 ± 0.4 (β-actin), respectively. The amount of mRNA was normalized to that of β-actin using the ΔCt method, as described in the manufacturer’s protocol. Data are expressed as mean ± SD (n = 6). One-way ANOVA with repeated measures was used for the comparison. Because the initial P value was less than 0.05, Scheffe’s test was used to determine the significance between groups. *p < 0.05 versus control.