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a: Thin layer chromatography of mycolic acid (MA) subclasses. The TLC plate was developed with n-hexane-diethyl ether (80:20, v/v; four runs) and visualized by spraying with phosphomolybdic acid. b–d: MALDI-TOF mass spectra of MA from M. bovis BCG Tokyo 172, α-MA (b), methoxy-MA (c), and keto-MA (d). e: The chemical structure of MA subclass derived from M. bovis BCG Tokyo 172.
