Fig 3.

a, b: Internalization of liposomal MA into bladder cancer cell lines. Cells of the human bladder cell line T24 and the murine bladder cell line MB49 were incubated with liposome labeled with the green fluorescence marker NBD-cholesterol for the indicated amount of time. Internalization of the liposome was assessed by (a) laser confocal microscopy of T24 cells and (b) flow cytometry of MB49 cells (%). c: The cellular association of NBD-labeled liposomes with murine bladder cancer cells analyzed by flow cytometry. Black line: MB49 without liposome. Blue line: Liposome without dendrimer. Red line: Liposome with dendrimer D22. The experiment was repeated 8 times. d: The direct cytotoxicity of MA liposome against MB49 cells. MB49 cells were incubated with liposome containing MA, liposome without MA, or PBS for 48 h in medium without FBS. Cell viability was measured by the WST-8 method. Values represent the absorbance ratio vs. absorbance at 0 h, and were calculated as the mean±SEM of at least three different experiments (P <0.05).