Fig 4.

a: Design of the in vivo study. Mice were subcutaneously administered a mixture of reagents (PBS, liposome with MA, or liposome without MA) and murine bladder cancer cells (MBT-2 or MB49). Each reagent was then subcutaneously administered on days 3, 5, and 7. Each of these experiments was repeated 5 times and showed similar results. b: C3H/HeN mice were administered MBT2 cells and treated with each reagent. At day 25, the growth of the MBT2 tumors was suppressed by treatment with Lip-kMA or Lip-mMA compared to PBS treatment (n = 8–9, p = 0.023, 0.038, respectively). The values are the mean±SEM. *p<0.05, Dunnett's test. c: The growth of the tumors was recorded. C57BL/6J mice (n = 5 or 6 for each arm) were administered MB49 cells and treated with each reagent. The growth of the MB49 tumors was suppressed by treatment with Lip-kMA compared to treatment with PBS (day 28, p = 0.028). The activities of Lip-aMA and Lip-mMA were not significant (day 28, p = 0.086, p = 0.16, respectively). The values are the mean±SEM. *p<0.05. Dunnett's test.