Table 6. Flux variability analysis (FVA) to show the effect of streptomycin on WT.
| Subsystem | Reaction ID | Reaction Formula | WT | WT+strep |
|---|---|---|---|---|
| TCA Cycle | AKGDH | coa_c + nad_c + akg_c -> co2_c + nadh_c + succoa_c | 0.05 | 1.10 |
| FUM | mal__L_c < = > fum_c + h2o_c | -5.31 | -3.14 | |
| MDH | nad_c + mal__L_c < = > h_c + nadh_c + oaa_c | 5.72 | 2.04 | |
| Oxidative phosphorylation | cytochrome oxidase bo3 ubiquinol-8 | 2.5 h_c + 0.5 o2_c + q8h2_c -> h2o_c + 2.5 h_e + q8_c | 31.22 | 10.03 |
| Pyruvate metabolism | PFL | accoa_c + for_c < = > coa_c + pyr_c | -10.84 to 0.23 | -1.88 to 1.1 |
| PPS | atp_c + h2o_c + pyr_c -> h_c + pi_c + pep_c + amp_c | 0.0002 | 0.15 | |
| Purine metabolism | ATP carbamate phosphotransferase | atp_c + co2_c + nh4_c < = > h_c + adp_c + cbp_c | 0.17 | 1.10 |
| Folate biosynthesis | MTHFD | nadp_c + mlthf_c < = > nadph_c + methf_c | 0.41 | 1.10 |
| FTHFD | h2o_c + 10fthf_c -> h_c + for_c + thf_c | 0.22 | 1.10 | |
| Glutamate metabolism | ASPTA | asp__L_c + akg_c < = > oaa_c + glu__L_c | -0.63 | -1.10 |
| Glycine, Serine and Threonine metabolism | PSERT | akg_c + pser__L_c < = > glu__L_c + 3php_c | -3.54 | -1.10 |
| GHMT | gly_c + h2o_c + mlthf_c < = > ser__L_c + thf_c | -0.46 | -1.10 | |
| Arginine and proline metabolism | PRO1x | h_c + nadh_c + 1pyr5c_c -> nad_c + pro__L_c | 0.35 | 0.002 |
| SOTA | akg_c + sucorn_c < = > sucgsa_c + glu__L_c | 4e-5 | 1.10 | |
| SGSAD | h2o_c + nad_c + sucgsa_c -> 2 h_c + nadh_c + sucglu_c | 4e-5 | 1.10 | |
| SGDS | h2o_c + sucglu_c < = > succ_c + glu__L_c | 4e-5 | 1.10 | |
| AST | arg__L_c + succoa_c -> h_c + coa_c + sucarg_c | 4e-5 | 1.10 | |
| Urea cycle and metabolism of amino groups | ARGSL | argsuc_c -> fum_c + arg__L_c | 0.07 | 1.10 |
| ARGSS_1 | atp_c + asp__L_c + citr__L_c -> ppi_c + argsuc_c + amp_c | 0.07 | 1.10 | |
| AGGPR | nadph_c + acg5p_c -> pi_c + nadp_c + acg5sa_c | 0.08 | 1.10 | |
| OCBT | cbp_c + orn_c -> 2 h_c + pi_c + citr__L_c | 0.07 | 1.10 | |
| ORNTAC | glu__L_c + acorn_c < = > orn_c + acglu_c | 0.08 | 1.10 | |
| ACGK | h_c + atp_c + acglu_c -> adp_c + acg5p_c | 0.08 | 1.10 | |
| ACOTA | glu__L_c + acg5sa_c -> akg_c + acorn_c | 0.08 | 1.10 | |
| Cyanoamino Metabolism | glycine:acceptor oxidoreductase | gly_c + 2 nadph_c -> co2_c + 2 nadp_c + hcn_c | 0.28 | 1.10 |
| cyn_rxn6 | hcn_c -> acybut_c | 0.28 | 1.10 | |
| NH4+ Exchange | nh4_e < = > | -6.28 | 1.09 |
For reaction details and color code for the FVA category refer to S1 Table.