Fig 3. Effects of combined AZD8999 and fluticasone propionate on inflammatory mediators in neutrophils from healthy and COPD patients.

Peripheral blood neutrophils from healthy controls and COPD patients were incubated with AZD8999, fluticasone propionate (Fluti) or both at non-effective concentrations for 1 h before they were stimulated with lipopolysaccharide (LPS) for 6 h. IL-8 (A), MMP9 (B), IL-1β (C) and GM-CSF (D) cytokine release was measured in cell supernatants. The results are expressed as the median with interquartile range (n = 3–5 each for cells from healthy controls and COPD patients in independent experiments with triplicate samples). Kruskal-Wallis test was followed by a Dunn’s post-hoc test. *p < 0.05 vs. control unstimulated cells; ⊥ p < 0.05 vs. monotherapy; #p < 0.05 vs. treatment without propranolol.