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. 2019 Jan 4;14(1):e0210188. doi: 10.1371/journal.pone.0210188

Fig 5. Effects of AZD8999, fluticasone propionate and combinations on lipopolysaccharide-induced corticosteroid modulators.

Fig 5

(A-C) Human peripheral blood neutrophils isolated from healthy and COPD patients were incubated with AZD8999, fluticasone propionate (Fluti) or a combination thereof for 1 h, and stimulated with lipopolysaccharide (LPS) for 6 h. Molecular corticosteroid modulators were quantified by RT-PCR using the 2−ΔCt method, with expression of the housekeeping gene GAPDH serving as an internal control. (D) PI3Kδ activity in human neutrophils from COPD patients. Peripheral blood neutrophils from COPD patients were incubated with AZD8999 or LY294002 for 1 h and stimulated with LPS for 30 min. The results are expressed as the median with interquartile range (n = 3 COPD cell populations in independent experiments run in triplicate). Kruskal-Wallis test was followed by a Dunn’s post-hoc test. *p < 0.05 vs. control unstimulated cells; #p < 0.05 vs. LPS-stimulated cells.