FIGURE 2. The effects of neuropeptide treatment on phagolysosome formation.
The isolated magnetic bead–containing vesicles were immunoblotted for Rab5 and LAMP1. The ratio of LAMP1 to Rab5 was calculated from the intensity of the bands. Presented are (A) a representative immunoblot and (B) the mean 6 SEM of the relative ratio of LAMP1/Rab5 from four independent experiments. *There is a significant (p = 0.03) suppression in the expression of LAMP1 to Rab5 in the macrophages cotreated with α-MSH and NPY. This demonstrates suppression in the formation of phagolysosomes.