FIGURE 6. Effects of neuropeptide treatment on Ia^d expression on macrophages.

The α-MSH/NPY–cotreated macrophages were fixed and stained for surface expression of Ia^d and analyzed by flow cytometry. (A) Repre-sentative histogram of three independent experiments with the gate set to exclude ˃.95% of isotype-stained cells. (B) The bar graph presents the relative intensity of Ia^d expression on untreated and α-MSH/NPY– cotreated macrophages given opsonized nonfluorescent bioparticles to phagocytize, and resting macrophages. No significant difference was seen between activated macrophages treated or untreated in Ia^d ex-pression. This demonstrates that MHC II expression is not altered in the treated macrophages.