REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
unconjugated purified goat anti-human IgG-Fc | Bethyl | Cat#A-80–104; RRID:AB67060 |
donkey anti-goat IgG (H+L) cross-adsorbed secondary antibody conjugated to Alexa Fluor 488 | Thermo Fisher Scientific | Cat#A-11055; RRID:AB_2534102 |
Purified Human IgG/Kappa | Bethyl | P80–111 |
anti-human Kappa light chain antibody conjugated to horseradish peroxidase | Bethyl | Cat#A80–115P; RRID:AB_67091 |
anti-human IgG (H+L) conjugated to horseradish peroxidase | Sigma Aldrich | SAB3701359 |
Bacterial and Virus Strains | ||
Mouse-adapted Zaire Ebolavirus (EBOV) | Public Health Agency of Canada | N/A |
Chemicals, Peptides, and Recombinant Proteins | ||
GeneJammer | Agilent | 204130 |
optimal cutting temperature (O.C.T) medium | Fisher Scientific | 23–730-571 |
ProLong Gold Antifade reagent with DAPI | Thermo Fisher Scientific | P36931 |
SIGMAFAST OPD | Sigma Aldrich | P9187 |
Ebola virus Glycoprotein (strain Mayinga 1976) | Sino Biological | 40304-V08B1 |
mAb 5.6.1A2 | Emory University | Dr. Rafi Ahmed |
mAb 15784 | Bornholdt et al. (2) | KU602185 KU602186 |
Experimental Models: Cell Lines | ||
Human embryonic kidney (HEK) 293 T cells | ATCC | Cat#ATCC #CRL-3216; RRID:CVCL_0063 |
African green monkey Vero E6 cells | ATCC | Cat#ATCC #CRl-1586; RRID:CVCL_0574 |
Experimental Models: Organisms/Strains | ||
BALB/c mouse | Charles River Labs | 028 |
Recombinant DNA | ||
DMAb DNA | This paper | N/A |
Software and Algorithms | ||
SPSS | IBM | N/A |
GraphPad Prism 7.0 | GraphPad Software | N/A |