Figure 9. Munc18-1 phosphomimetic and disease mutations altered chaperoned SNARE assembly.

(A) FECs for Munc18-1 mutations with 0 nM (#3) or 60 nM (others) SNAP-25B. See also Figure 9—source data 1. (B) Extension-time trajectories at the indicated constant mean forces, some of which (e and f) are extracted from panel A. In panels a, g, and h, no template complex formation is observed. See also Figure 9—source data 2.