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. Author manuscript; available in PMC: 2019 Aug 3.
Published in final edited form as: Sci Immunol. 2018 Aug 3;3(26):eaao4747. doi: 10.1126/sciimmunol.aao4747

Figure 3: DNase instillation altered levels of NETs, but not neutrophilia.

Figure 3:

Mice were subjected to DNase (i.n., 6 hours post-sensitization with HDM/LPS) or PBS control and tissues were harvested at the end of the sensitization period (day 3). (A) Picogreen assays showing the amount of DNA in BALFs (n = 5 mice). (B) Western blot showing hyper- citrullinated histone H3 (top panel) and Ponceau S stain as loading control (bottom panel) (n = 5 mice). (C-E) To assess inflammatory responses, BAL was performed at the end of the allergen (HDM) challenge on protocol day 15. (C) BALF total cell count and (D, E) leukocyte differential (% total leukocytes and cell count) were enumerated (n = 3 mice). *P<0.05, **P<0.01 by Student’s t-test.