Figure 3:

Cell viability rescue data. HCT116 p53^+/+ and HCT116 p53^−/− cells were co-treated with indicated concentrations of (left) F10 or (right) 5-FU along with indicated nucleoside (uridine = 1 mmol/L, thymidine = 80 μmol/L) for 48 h. Cell viability was determined using Promega’s CellTiter-Glo ATP assay after 48 h and calculated following blank subtraction relative to respective controls. Experiments done in triplicate ± SEM. P-values calculated using GraphPad Prism (n = 4; *P ≤ 0.05, ****P ≤ 0.0001)