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. 2018 Nov 15;104(2):581–594. doi: 10.1210/jc.2018-01493

Figure 2.

Figure 2.

CD34+lymphocyte-specific protein-1 (LSP-1)+TSHR+ fibrocytes can be identified in the orbital tissue of patients with TAO but are absent in tissues from healthy donors. (A) CD34 expression (arrows, green; fluorescein isothiocyanate) in TAO-derived tissue (inset shows negative control staining). (B) Absent CD34 expression in healthy tissue (inset shows positive staining control). (C) LSP-1 expression in TAO-derived tissue (red, arrows; nuclei counterstained with 4′,6′-diamidino-2-phenylindole; inset shows negative control). (D) Absence of LSP-1 expression in healthy tissue (inset shows negative control). (E) CD31 expression in disease-derived tissue is limited to vascular endothelium (red, arrows). (F) Hematoxylin and eosin–stained consecutive thin sections of the same orbital tissue (original magnification, ×40). (G) Fibrocytes present in orbital tissue from patients with TAO coexpress CD34 and TSHR as demonstrated by confocal microscopy. Thin-sectioned tissue from a donor with TAO was stained with anti-CD34 (green) and anti-TSHR (red) antibodies. Nuclei were counterstained with 4′,6′-diamidino-2-phenylindole. The inset contains a negative staining control. Reproduced with permission from Douglas RS, Afifiyan NF, Hwang CJ, et al. Increased generation of fibrocytes in thyroid-associated ophthalmopathy. J Clin Endocrinol Metab 2010; 95(1):430-438.