Figure 5.

(A–D) Higher levels of (A) AIRE, (B) MHC-II, (C) Tg, and (D) TSHR in fibrocytes than OFs from patients with GD (GD-OFs). (Left panels) RNA was reverse transcribed and subjected to real-time PCR. (Right panels) Cells were stained with the labeled antibodies for flow cytometry. mRNA data were normalized to their respective glyceraldehyde-3-phosphate dehydrogenase levels and are expressed as the mean ± SD. Data from flow analysis denote fluorescence intensity compared with isotype controls. ***P < 0.001. iso, isotype control. Reproduced with permission from Fernando R, Diniz Grisolia AB, Lu Y, et al. Slit2 modulates the inflammatory phenotype of orbit-infiltrating fibrocytes in Graves’ disease. J Immunol 2018; 200(12):3942-3949. Copyright 2018 The American Association of Immunologists, Inc.