Extended Data Fig. 10 |. Effect of TET2 knockdown on the cytotoxic machinery of CAR T cells.

a, Flow cytometry plots showing the frequency of TET2 knockdown or control CAR T cells expressing CD107a (a marker of cytolysis) following CD3 and CD28 or CAR-specific stimulation (left). Summarized data from analysis of CAR T cells manufactured from n = 6 different healthy donors is shown (right). b, Representative histograms illustrating expression levels of granzyme B and perforin in CAR T cells in the setting of TET2 inhibition as compared to its counterpart control (left). Pooled data from CAR T cells of n = 5 healthy donors are summarized on the right. c, Cytotoxic capacity of CTL019 cells (transduced with a TET2 or scrambled control shRNA) after overnight co-culture with luciferase-expressing OSU-CLL (left) or NALM-6 (right) cells. Untransduced T cells were included as an additional group to control for non-specific lysis. P values were determined using a two-tailed, paired Student’s t-test. All data were pooled from three independent experiments.