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. Author manuscript; available in PMC: 2019 Jan 4.
Published in final edited form as: ACS Chem Biol. 2015 Oct 14;11(3):734–741. doi: 10.1021/acschembio.5b00709

Figure 4.

Figure 4.

Real-time kinetic monitoring of histone acetylation provides insights into the mechanism of KAT inhibitors. (a) Kinetic monitoring of Gcn5-catalyzed acetylation in the presence or absence of inhibitors.(b) Ratio of acetylation rates at early (0–25 min) and late (65–90 min) time points. High ratios indicate reduced reaction rates at later time points, suggestive of time-dependent inhibition. (c) Garcinol inhibition of Gcn5 and p300 is sensitive to the concentration of the detergent Triton-X. (d) Tat-CoA inhibition of p300 and Gcn5 is not sensitive to detergent. (f) Tat-CoA inhibits acetylation in cells.