Fig E5.

Chemokines can be used to stimulate human blood cells in a time-dependent manner. (A) CD3⁺ T cells and (B) Lineage⁻, CD123⁻, CD294⁺ ILC2s were identified from within a single-cell, lymphocyte gate. C, CCR4 expression on T cells (gray) and ILC2s (red) with gates used to select positive and negative populations overlaid. Phalloidin staining was analyzed for (D) CCR4⁻ T cells, (E) CCR4⁺ T cells, and (F) CCR4⁺ ILC2s at the indicated time points. Receptor response was determined for (G) chemokine receptor–positive T cells and (H) chemokine receptor–positive ILC2s at each time point by normalizing the phalloidin MFI to that observed on CCR4⁻ cells. Data shown are mean ± SEM of 5 independent donors. MFI, Mean fluorescence intensity; SSC-A, side scatter-area.