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Immobilization of proteins and amino acid sequences. (a) Dye-labeled protein molecules are immobilized on a polyethylene glycol (PEG)-coated glass surface via a biotinstreptavidin-biotin linkage. (b) Dyes are attached to the cysteine residues (red) and a biotin molecule to the lysine residue (blue) in the 15-residue biotin accepting sequence (AviTag). α3D and gpW were labeled with Alexa 488 and Alexa 594 and the WW domain was labeled with Alexa 488 and Atto 647N.
