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. Author manuscript; available in PMC: 2019 Jan 4.
Published in final edited form as: J Phys Chem B. 2016 Jan 26;120(4):680–699. doi: 10.1021/acs.jpcb.5b11351

Figure 4.

Figure 4.

Bin time dependence of the FRET efficiency histograms and 2D histograms of donor fluorescence lifetime vs. FRET efficiency of gpW ([GdmCl] = 2.5 M) constructed from the donor (a) and acceptor (b) delay times. Compared to the data of α3D, two peaks are not clearly distinguishable at the shortest bin time of 0.5 ms both in the 1D and 2D histograms due to the faster kinetics. The parameters used in the plots were obtained as explained in Figure 3.