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Bin time dependence of the FRET efficiency histograms and 2D histograms of donor fluorescence lifetime vs. FRET efficiency of the WW domain ([GdmCl] = 2 M) constructed from the donor (a) and acceptor (b) delay times. Two peaks are not distinguishable at all bin times both in the 1D and 2D histograms due to the much faster kinetics compared to the shortest bin time, 0.5 ms. The parameters used in the plots were obtained as explained in Figure 3.
