Figure 8.
The relative donor lifetime (τD/τD0) obtained from the maximum likelihood analysis of the photon trajectories including donor delay time information with the two-state model (2-state/D, orange), four-state model (4-state/D, purple), and modified two-state model with acceptor blinking (2-state/Db, black). Parameters are also listed in Table 3. The lifetimes in the folded (circle) and unfolded (square) states in the 2-state/D and 4-state/D analyses were determined by fixing the pre-determined FRET efficiencies, relaxation rates, folded fraction, and acceptor blinking parameters from the 2-state and 4-state analyses, respectively. In the 2-state/Db analysis, the fraction of the population in the acceptor bright state (pb0) was determined together with the lifetime parameters. (a) Experimental parameters. (b) Parameters obtained from the recolored data using the experimental 4-state/Ds parameters (dashed lines).