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. Author manuscript; available in PMC: 2020 Jan 3.
Published in final edited form as: Mol Cell. 2018 Nov 29;73(1):107–118.e4. doi: 10.1016/j.molcel.2018.10.031

Figure 2. Pol II cleavage activity antagonizes termination at 3’ ends of genes.

Figure 2.

(A)Expression of TFIISDN causes early transcription termination. Note the early drop in Pol II, Ser2-P, and TFIIS ChIP signals at gene 3’ ends when TFIISDN is expressed.

(B)Metaplots of anti-pol II ChIP-seq signals near poly(A) sites (pAS) (50 bp bins) for genes >1 kb long and separated by >5 kb. The mean signal was calculated for each individual replicate. The replicates were then averaged with the shaded region representing the SEM for three replicates. RPKM, reads per kb per million mapped reads.

(C) Metaplots of anti-CTD Ser2-P pol II signals as in B for one replicate.

(D)Metaplots of mNET-seq signals as in B for two replicates.

(E) Metaplots of WT TFIIS and TFIISDN ChIP-seq signals (50 bp bins) normalized to a yeast spike-in as in Fig. 1F for genes shown in B.