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. Author manuscript; available in PMC: 2020 Jan 3.
Published in final edited form as: Mol Cell. 2018 Nov 29;73(1):107–118.e4. doi: 10.1016/j.molcel.2018.10.031

Figure 6. Pol II cleavage activity is required to rapidly activate gene expression.

Figure 6.

(A) TFIISDN impairs transcriptional activation of hypoxia inducible genes in response to DMOG. Metaplots of pulse-labelled nascent RNA-seq (Bru-seq) signals for cells treated +/−2 mM DMOG for 16 hours. Data were plotted for genes with increased signal +DMOG (p-value < 0.01). The mean signal and SEM was plotted for two biological replicates as in Fig. 2A. Negative values correspond to anti-sense signal.

(B) qRT-PCR showing the fold change in mRNA abundance for hypoxia-responsive transcripts in cells treated with 2 mM DMOG for indicated times. Error bars represent the SEM for at least two biological replicates.

(C) Lactate/pyruvate ratios were determined by UHPLC-MS in uninduced and TFIISDNexpressing cells +/−2 mM DMOG for 16 hrs. ** p < 0.01, *** p < 0.001 ANOVA.

(D) qRT-PCR showing the fold change in LDHA mRNA as in B.

(E) qRT-PCR showing the fold change in heat shock mRNA after incubation at 42° as in B.