Figure 2:

CHRFAM7A expression decreases ligand binding to the α7nAchR in PC12 cells. Rat PC12 cells were transduced with human CHRFAM7A to quantify changes in α-BTX binding using flow cytometry. A. Gating strategy to identify the GFP⁺CHRFAM7A⁺ population in vector and CHRFAM7A-transduced cells. B. Representative histogram demonstrating decreased α-BTX binding in CHRFAM7A transduced PC12 cells compared to vector. C. Cell preincubated with the α7nAchR ligand nicotine (50μM) to prevent α-BTX binding to functional α7nAch receptors. D. Quantification of all flow cytometry experiments showing that the introduction of uniquely human CHRFAM7A decreases α-BTX binding to PC12 cells. * p<0.05.