Figure 1.

Effect of anti-GD2 antibody dinutuximab (dinutux) and aNK cells on neuroblastoma cells in vitro. Calcein AM-based digital imaging microscopy assay showing the viability of human neuroblastoma cell lines SH-SY5Y-Fluc, CHLA-255-Fluc and CHLA-136-Fluc following treatment with dinutuximab alone (100 ng/ml), aNK cells alone, or their combination for six hours. For each cell line, 3×10⁴ NB cells were seeded per well of a 96-well plate, along with 1.5×10⁴ aNK cells as described in Materials & Methods (E:T ratio 1:2). Each condition contained six replicates and each experiment was performed a minimum of two times. Comparisons were made between groups using ANOVA and post-hoc Tukey’s HSD tests.