Table 1.
Percentage of on‐target mutation of T0 maize plants transformed with different CRISPR nucleases and guide RNAs.a
| Construct ID | CRISPR/Cas Systems | No. of Events | No. of plantlets Analysed | Genotypeb | Total Efficiency | ||||
|---|---|---|---|---|---|---|---|---|---|
| Wild Type | Mosaic | Heterozygous | Biallelic | Homozygous | |||||
| A842B | LbCas12a + crRNA1 | 4 | 20 | 40% (8) | 10% (2) | 45% (9) | 5% (1) | 0% (0) | 60% (20) |
| A843B | LbCas12a + crRNA2 | 6 | 23 | 100% (23) | 0% (0) | 0% (0) | 0% (0) | 0% (0) | 0% (23) |
| A844B | zCas9 + gRNA1 | 7 | 27 | 0% (0) | 0% (0) | 37% (10) | 18.5% (5) | 44.4% (12) | 100% (27) |
| A845B | zCas9 + gRNA2 | 9 | 31 | 9.7% (3) | 0% (0) | 12.9% (4) | 58.1% (18) | 19.4% (6) | 90.3% (31) |
a
Numbers in parentheses represent number of plants analysed.
b
Mosaic, three or more mutant sequences in a single plant; Heterozygous, wild‐type sequence and one mutant sequence; Biallelic, two different mutant sequences; Homozygous, one mutant sequence without wild‐type allele.