. 2019 Jan 4;9(1):32. doi: 10.1007/s13205-018-1555-x

Table 4.

Keratinase activities and biodegradation of HMPF and HMFF containing different concentrations of heavy metals by immobilised cells of Alcaligenes sp. AQ05-001

HMPFs type	Concentrations of heavy metals (ppm)
	1		5		10		15		20		25		30
	KA (U/mL)	FD (%)	KA (U/mL)	FD (%)	KA (U/mL)	FD (%)	KA (U/mL)	FD (%)	KA (U/mL)	FD (%)	KA (U/mL)	FD (%)	KA (U/mL)	FD (%)
Hg	98.6 ± 0.4^c	100 ± 0^a	96.9 ± 1.4^e	98 ± 0.2^a	15.4 ± 1.4^g	14.7 ± 3.1^f	1.9 ± 2.4^h	0 ± 0^f	0.1 ± 0^h	0 ± 0^e	0 ± 0^d	0 ± 0^e	0 ± 0^e	0 ± 0^c
Zn	101.3 ± 1.1^c	100 ± 0^a	100 ± 0^e	98 ± 0.4^a	33.1 ± 0.8^f	43.2 ± 1.5^e	11.4 ± 0.1^g	7 ± 0^e	0.4 ± 0^h	0 ± 0^e	0 ± 0^d	0 ± 0^e	0 ± 0^e	0 ± 0^c
Pb	100.3 ± 1.7^c	92 ± 0.6^b	72.8 ± 1.2^f	93 ± 1.8^a	29.3 ± 2.1^f	21 ± 0.9^d	17.6 ± 0.8^f	15 ± 0^d	9.5 ± 0.5^g	10 ± 0^d	2.4 ± 1.4^d	0 ± 0^e	0 ± 0^e	0 ± 0^c
Ni	103.1 ± 1.9^c	99 ± 0.1^a	67.3 ± 2.2 ^g	78 ± 2.3^b	55.6 ± 0.5^e	56 ± 2.3^c	22.5 ± 1.2^e	32 ± 2.6^c	0 ± 0^h	10 ± 0^d	0 ± 0^d	0 ± 0^e	0 ± 0^e	0 ± 0^c
As	118.4 ± 0.5^b	99 ± 0.1^a	108 ± 1.8^d	97 ± 1.3^a	73.9 ± 0.8^d	81 ± 1.4^b	71.4 ± 2.5^d	78.6 ± 1.8^b	34.1 ± 2.1^f	17 ± 0.3^c	0.5 ± 0.1^d	0 ± 0^e	100 ± 0^a	0 ± 0^c
Cd	116.3 ± 0.8^b	100 ± 0^a	119 ± 0^bc	100 ± 0^a	112.7 ± 1.1^c	100 ± 0^a	103.9 ± 2.3^c	98.5 ± 1.7^a	43.2 ± 0.9^e	76 ± 0.4^b	53.2 ± 1.2^c	10 ± 0^d	0 ± 0^e	0 ± 0^c
Co	120.4 ± 0.4^ab	100 ± 0^a	119 ± 0^bc	100 ± 0^a	121.7 ± 1.0^ab	100 ± 0^a	120.2 ± 0.4^a	100 ± 0^a	114.3 ± 2.3^c	100 ± 0^a	90.6 ± 1.8^b	94 ± 1.2^c	90.4 ± 1.6^c	91 ± 0^c
Cu	119.1 ± 3.1^ab	100 ± 0^a	121 ± 1.3^b	100 ± 0^a	117.9 ± 0.8^bc	100 ± 0^a	115.5 ± 0.9^b	100 ± 0^a	83.1 ± 1.1^d	96 ± 1.0^a	54.3 ± 0.8^c	71.9 ± ^b	49.3 ± 2.3^d	54 ± 1.2^b
Ag	122.7 ± 2.4^a	100 ± 0^a	129 ± 0.7^a	`100 ± 0^a	123.8 ± 0.4^a	100 ± 0^a	116.9 ± 0.3^b	100 ± 0^a	121.3 ± 1.4^a	100 ± 0^a	118.4 ± 0.6^a	100 ± ^a	108.8 ± 3.2^b	97 ± 1.1^a
Control (HMFF)	117.1 ± 0.4^ab	98.2 ± 1.3^a	117.3 ± 0.7^c	99.6 ± 0.5^a	117.6 ± 0.5^bc	100 ± 0.0^a	117.5 ± 0.8^ab	100 ± 0.0^a	117.9 ± 1.3^b	97.6 ± 1.1^a	117.6 ± 1.3^a	98.6 ± .08^a	118.2 ± 0.8^a	98.7 ± 0.8^a

Results are expressed as mean ± SD. Mean values that do not share a letter within a column are significantly different (p < 0.05)

KA Keratinase activities, FD feather degradation, ppm part per million