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. 2019 Jan 4;2:11. doi: 10.1038/s42003-018-0255-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Matriptase is responsible for Epcam cleavage. a Knockdown efficacy of matriptase (encoded by St14 gene) shRNA in organoids from Spint2^LoxP/LoxPCreERT2 mice. RNA was extracted from mock-transfected control (cont) and matriptase shRNA lentivirus vector-transfected (KD) organoids and matriptase mRNA levels were verified by quantitative RT-PCR. The 25th and 75th percentile (boxes) and the median (bold line within the boxes) are plotted. Circle represents the value of each measurement. *p = 0.029 (Student t-test; n, 4). b Control and matriptase-knockdown organoids were treated with 4-OHT (1 μM) or vehicle only (ethanol) for 28 h. Immunoblot analysis of Epcam and claudin-7 (left panel) and representative morphology of organoids (right panel) are shown. c Dose-dependent effects of matriptase-selective inhibitor (ZFH7185-8) on Epcam cleavage and claudin-7 in Spint2^LoxP/LoxPCreERT2 organoids with or without 4-OHT treatment (28 h). Hepsin-selective inhibitor (ZFH7185-3) was also used as a control (lower panel). Representative photos of organoids are also shown