Table 4.

Paclitaxel and baccatin III content [µg/g DW] in two lines of Taxus × media hairy roots cultivated under various culture conditions

Culture variant	ATMA hairy root line (carrying TXS transgene)		KT hairy root line (without TXS transgene)
Day of culture	Paclitaxel	Baccatin III	Paclitaxel	Baccatin III
Control
14	nd	nd	18.33 ± 5.61i	nd
28	nd	nd	22.01 ± 7.92	nd
35	nd	nd	23.78 ± 1.84n,o,u	nd
42	nd	nd	28.80 ± 3.30i,j,k,p,s,w	nd
49	nd	nd	12.52 ± 1.89i,j,x	nd
Unelicited cultures with PFD-aerated
28	nd	nd	50.15 ± 23.49	nd
35	nd	nd	20.75 ± 19.48o,u	nd
42	nd	nd	17.74 ± 3.03k,s,w	nd
49	nd	nd	21.45 ± 8.55m,x	nd
Unelicited cultures with PFD-degassed
28	nd	nd	14.70 ± 5.50	nd
35	nd	nd	14.16 ± 2.13n,o,u	nd
42	nd	nd	18.69 ± 6.24l,s	nd
49	nd	nd	64.91 ± 25.34l,m,x	nd
Single-elicited cultures without PFD
35	780.43 ± 136.74a,e,*	nd	164.62 ± 9.12o,u,*	nd
42	1570.14 ± 134.2a,b,d,g	nd	nd	nd
Twice-elicited cultures without PFD
42	1259.86 ± 117.93b,d,g,*	nd	22.87 ± 1.58p,s,w,*	nd
49	1611.65 ± 228.96h	nd	nd	nd
Single-elicited cultures with PFD-aerated
35	1292.21 ± 99.77c,e,*	nd	51.56 ± 15.29r,*	nd
42	2136.88 ± 266.83c,d,g,*	2.76 ± 0.62	470.08 ± 25.15r,s,w,*	nd
Twice-elicited cultures with PFD-aerated
42	1246.07 ± 157.95g,*	1.91 ± 0.39	320.51 ± 33.24s,*	nd
49	1641.83 ± 509.17	6.58 ± 2.20	nd	nd
Single-elicited cultures with PFD-degassed
35	1038.42 ± 288.28f,*	nd	67.12 ± 7.04t,u,*	nd
42	2473.29 ± 263.85f,g,*	2.32 ± 0.32	3.94 ± 1.17s,t,w,*	nd
Twice-elicited cultures with PFD-degassed
42	1577.22 ± 347.18g,*	2.62 ± 0.72	401.02 ± 164.25*	nd
49	2056.19 ± 79.60h,*	4.70 ± 1.39	249.08 ± 62.84x,*	nd

Data presents mean ± SD. The same letters within columns indicate means statistically different (p < 0.05), while “*” indicates means statistically different (p < 0.05) within rows